Product: AP02353PU-NAKT1 / PKB pSer473 antibody
Quantity: 0.1 ml
Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Rabbit
Isotype:
CAS NO: 1215085-92-9 Product: GLPG0492
Shipping to: Worldwide
Immunogen: Peptide Sequence around the phosphorylation site of Ser473 (Q-F-Sp-Y-S) derived from Human AktGeneID:207
Application: Western Blot: 1/500-1/1000.Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Background: The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases.Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulat
Concentration: 1.0 mg/ml
Storage: Store the antibody (in aliquots) at -20°C.Avoid repeated freezing and thawing.Shelf life: One year from despatch.
Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
Preservatives:
State: Liquid purified Ig fraction.Aff – Purified
Specifictiy: This antibody detects endogenous levels of Akt only when phosphorylated at Serine 473.
