Product: AP02354PU-NAKT1 / PKB pThr308 antibody
Quantity: 0.1 ml
Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Rabbit
Isotype:
CAS NO: 113559-13-0 Product: E-4031
Shipping to: Worldwide
Immunogen: Synthetic phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C).GeneID:207
Application: Western blot: 1/500 – 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.Immunohistochemistry on paraffin sections: 1/50 – 1/100.Immunofluorescence: 1/100 – 1/200.
Background:
Concentration: 1.0 mg/ml
Storage: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer
Preservatives:
State: Liquid purified Ig fractionAff – Purified
Specifictiy: Akt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308.
