Product: AP09293PU-NActin beta / ACTB (359-368) (Loading Control) antibody
Quantity: 0.2 mg
Synonyms: Actin cytoplasmic 1, Beta-Actin
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Rabbit
Isotype: IgG
CAS NO: 1338545-07-5 Product: OTS-964
Shipping to: Worldwide
Immunogen: Synthetic peptide corresponding to amino acids 359-368 of Human beta ActinGeneID:60
Application: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.Recommended Dilutions:ELISA: 1/10,000-1/40,000.Western Blot: 1/1,000-1/4,000.Immunofluorescence: 1/500-1/2,000.
Background: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as comp
Concentration: 1.02 mg/ml (by UV absorbance at 280 nm)
Storage: Store the antibody at -20°C.Dilute only prior to immediate use.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide.
Preservatives:
State: Liquid (sterile filtered) purified Ig fraction.Aff – Purified
Specifictiy: This antibody is directed against beta Actin protein.Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains.Paraformaldehyde fixation yields brighter staining than formalin or met
