Product: AP31793PU-N6xHistidine Epitope Tag (HHHHHH) antibody
Quantity: 0.1 ml
Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Chicken
Isotype: Ig
CAS NO: 479-13-0 Product: Coumestrol
Shipping to: Worldwide
Immunogen: 6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freunds adjuvants.After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were
Application: Immunoprecipitation.Recommended Dilutions: Use 1/5000-1/10000 as starting points for Immunoprecipitation and ChIPs.
Background: In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called “tags” can be used e.g. to determine the cellular localization or to quantify proteins. The polyhistidine “tag”
Concentration: 1.0 mg/ml (based on absorbance at 280 nm)
Storage: Store the antibody at -20°C in the dark.Avoid repeated freezing and thawing.Centrifuge vial before opening.Shelf life: one year from despatch.
Buffer System: Sodium Phosphate-Buffered (10 mM) isotonic (0.9%, w/v) Saline (PBS, pH 7.2) (50% by volume) with 50% Glycerol as an anti-freezing agent and 0.01% Thimerosal as preservative.
Preservatives:
State: Liquid purified IgY fractionAff – Purified
Specifictiy: This antibody recognizes the 6xHIS-Tag with high titre and high specificity in Immuoprecipitation.Quality Control: This antibody preparation was analyzed by Western blotting (at a dilution of 1/10000) using tissue extracts expressing a recombinant protein
