Product: SM1693PS6xHistidine Epitope Tag (HHHHHH) antibody
Quantity: 0.1 mg
Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG1
CAS NO: 136831-48-6 Product: RG13022
Shipping to: Worldwide
Immunogen: PAX6 transcription factor linked to Histidine tag.Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Application: ELISA.Immunoprecipitation.Immunhistology on Frozen Sections.Western blot (1/500-1/1000): This antibody is routinely tested on Histidine tagged recombinant protein.
Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Concentration: 1.0 mg/ml
Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: PBS, pH 7.4
Preservatives: 0.09% Sodium Azide
State: Liquid purified IgG fractionPurified
Specifictiy: This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It r
