Product: SM1872P5-Methyl Cytosine / 5-MeC antibody
Quantity: 0.1 mg
Synonyms: 5-MeCyd, 5-Methyl Cytidine, 5-Methylcytidine, 5MeC
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG1
CAS NO: 413611-93-5 Product: 10074-G5
Shipping to: Worldwide
Immunogen: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0Ag 14 myeloma cell line
Application: Flow Cytometry: Use at an assay dependent dilution. (Use 10 µl of working dilution to label 1,000,000 cells in 100 µl. (See Habib,M. et al. 1999)Membrane permeabilisation may be required for this application. Cell pretreatment before staining is described
Background: Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that is formed when Cytosine is attached to a Ribose ring (also known as a Ribofuranose) via a beta-N1-glycosidic bond.
Concentration: 1.0 mg/ml (at O.D. 280nm)
Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 10 mM PBS, pH 7.4
Preservatives: 0.01% Thimerosal
State: Liquid purified IgG fraction (<98% Gel electophoresis SDS PAGE)Purified
Specifictiy: This antibody recognizes the modified base 5-Methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNAmethylation is a DNA modification process, which is involved in the control of gene expression.Clone 33D3 has been developed to discriminate b
