Product: TA319275AKT1 / PKB pSer473 antibody
Quantity: 0.1 mg
Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation: Aff – Purified
Clonality: Polyclonal
Host: Rabbit
Isotype:
CAS NO: 170006-73-2 Product: FTI-277
Shipping to: Europe, USA/Canada
Immunogen: A synthetic peptide corresponding to the C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLHAA Sequence:C-H-F-P-Q-F-pS-Y-S-A-SGeneID:207Remarks:Human AKT1 protein, 480 aa, has a predicted MW of 55.7 kDa.
Application: Western blotting (1/200-1/1000).Immunohistochemistry (Formalin-Fixed Paraffin-Embedded Sections, 1/100-1/500) (No pre-treatment of sample is required).ELISA (1/15,000-1/60,000).Immunofluorescence.
Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transdu
Concentration: 0.75 mg/ml (by UV absorbance at 280 nm)
Storage: Store vial at -20°C prior to opening. This product is stable for one month at 2-8°C as an undiluted liquid. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature.D
Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative.
Preservatives:
State: Liquid (sterile filtered) purified Ig fraction.Aff – Purified
Specifictiy: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.This antibody is specific for phosphorylated AKT.Minimal reactivity occurs against non-phosphorylated AKT.
