Product: TA319562AKT1 / PKB pSer473 antibody
Quantity: 0.1 mg
Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG1
CAS NO: 942425-68-5 Product: PHA-767491 (hydrochloride)
Shipping to: Europe, USA/Canada
Immunogen: Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein, followed by hybridoma development.GeneID:207
Application: ELISA: 1/20,000.Immunoprecipitation.Immunofluorescence: 1/500-1/3,000.Western blot: 1/500-1/3,000. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein in the appropriate cell lysate or extract.Immunohistochemistry on For
Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal t
Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives: 0.01% (w/v) Sodium Azide
State: Liquid (sterile filtered) purified IgG fraction from concentrated Tissue Culture SupernatantPurified
Specifictiy: This phospho-specific monoclonal antibody reacts with Human and Mouse AKT pSer473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.Cross-reactivity with AKT2 and AKT3 has not been determined.
