Se without neglect. We modelled associations with four Vercirnon site levels of adjustment. First, we adjusted for exact age to allow for variations of several months at each follow-up. Second, we added adjustments for early-life factors. Third, we further adjusted for adult factors. Fourth, to assess whether any associations between child maltreatment and zBMI trajectory were independent of depressive ICG-001 biological activity symptom level, we further adjusted for time-varying depressive symptoms. Maltreatment associations with obesity risk from 7y to 50y were examined using logistic regression with robust standard errors to allow for correlated within-individual obesity measures. We tested the interaction between maltreatment and age which (when exponentiated) represents the proportional change in the odds ratio (OR) for obesity/y of age. An interaction between maltreatment and age2 was examined; it was significant and retained in the model for psychological abuse among females only. We used the four levels of adjustment described above. Sensitivity analyses were conducted. First, because childhood obesity was uncommon (<2.5 ) we repeated all analyses using !95th BMI or zBMI percentile at each age to test whether findings were influenced by obesity cut-offs. As expected, the 95th centile cut-offs were lower than obesity cut-offs for ages 7 to 23y, but higher than obesity cut-offs for ages 33 to 50y (Table 1 and S1 Table). Second, because some maltreatments were correlated (i.e. for physical and psychological abuse r = 0.5; in males, for sexual abuse with other abuse r = 0.3) we conducted additional analyses adjusting for correlated maltreatments. Where relevant we report these findings. From 17,638 participants enrolled at birth and immigrants by 7y (n = 378), 16,729 were alive and living in Britain at 7y; of these 16,639 participated (16,194 with data on neglect) at 7y or 11y. For childhood abuse, of 9,377 respondents at 45y, 9,315 provided relevant data. Multilevel models for participants with !1 BMI measure, 7y to 50y, were included: N = 15,424 for analysis of neglect at 7/11y; N 9310 for abuse. To minimize further data loss, missing covariates were imputed using MICE (multiple imputation chained equations) following current guidelines [22]. Imputation models included all model variables plus previously identified key predictors of missingness: i.e. cognitive ability and emotional status at 7y and class at birth [14]. Regression analyses were run across 10 imputed datasets. Imputed results were broadly similar to those using observed values; the former are presented. To facilitate comparison with our parallel studies of child maltreatment with height growth and pubertal timing[23,24] and with studies of BMI that show gender-specific results[7,8,25], all analyses were conducted for males and females separately.ResultsApproximately 12 of participants reported childhood abuse, psychological abuse was the most and sexual abuse the least common; 20 of participants had a childhood neglect scorePLOS ONE | DOI:10.1371/journal.pone.0119985 March 26,5 /Child Maltreatment and BMI TrajectoriesTable 2. Mean difference (95 CI) in BMI (kg/m2) and OR (95 CI) for obesity at each age by childhood maltreatment (unadjusted). 7y 11y 16y 23y Males Abuse Physical Psychological Sexual Neglect score !2 at 7 and/or 11y Abuse Physical Psychological Sexual Neglect score !2 at 7 and/or 11y Abuse Physical Psychological Sexual Neglect score !2 at 7 and/or 11y Abuse Physical Psychological Sexual.Se without neglect. We modelled associations with four levels of adjustment. First, we adjusted for exact age to allow for variations of several months at each follow-up. Second, we added adjustments for early-life factors. Third, we further adjusted for adult factors. Fourth, to assess whether any associations between child maltreatment and zBMI trajectory were independent of depressive symptom level, we further adjusted for time-varying depressive symptoms. Maltreatment associations with obesity risk from 7y to 50y were examined using logistic regression with robust standard errors to allow for correlated within-individual obesity measures. We tested the interaction between maltreatment and age which (when exponentiated) represents the proportional change in the odds ratio (OR) for obesity/y of age. An interaction between maltreatment and age2 was examined; it was significant and retained in the model for psychological abuse among females only. We used the four levels of adjustment described above. Sensitivity analyses were conducted. First, because childhood obesity was uncommon (<2.5 ) we repeated all analyses using !95th BMI or zBMI percentile at each age to test whether findings were influenced by obesity cut-offs. As expected, the 95th centile cut-offs were lower than obesity cut-offs for ages 7 to 23y, but higher than obesity cut-offs for ages 33 to 50y (Table 1 and S1 Table). Second, because some maltreatments were correlated (i.e. for physical and psychological abuse r = 0.5; in males, for sexual abuse with other abuse r = 0.3) we conducted additional analyses adjusting for correlated maltreatments. Where relevant we report these findings. From 17,638 participants enrolled at birth and immigrants by 7y (n = 378), 16,729 were alive and living in Britain at 7y; of these 16,639 participated (16,194 with data on neglect) at 7y or 11y. For childhood abuse, of 9,377 respondents at 45y, 9,315 provided relevant data. Multilevel models for participants with !1 BMI measure, 7y to 50y, were included: N = 15,424 for analysis of neglect at 7/11y; N 9310 for abuse. To minimize further data loss, missing covariates were imputed using MICE (multiple imputation chained equations) following current guidelines [22]. Imputation models included all model variables plus previously identified key predictors of missingness: i.e. cognitive ability and emotional status at 7y and class at birth [14]. Regression analyses were run across 10 imputed datasets. Imputed results were broadly similar to those using observed values; the former are presented. To facilitate comparison with our parallel studies of child maltreatment with height growth and pubertal timing[23,24] and with studies of BMI that show gender-specific results[7,8,25], all analyses were conducted for males and females separately.ResultsApproximately 12 of participants reported childhood abuse, psychological abuse was the most and sexual abuse the least common; 20 of participants had a childhood neglect scorePLOS ONE | DOI:10.1371/journal.pone.0119985 March 26,5 /Child Maltreatment and BMI TrajectoriesTable 2. Mean difference (95 CI) in BMI (kg/m2) and OR (95 CI) for obesity at each age by childhood maltreatment (unadjusted). 7y 11y 16y 23y Males Abuse Physical Psychological Sexual Neglect score !2 at 7 and/or 11y Abuse Physical Psychological Sexual Neglect score !2 at 7 and/or 11y Abuse Physical Psychological Sexual Neglect score !2 at 7 and/or 11y Abuse Physical Psychological Sexual.
