Proliferative disorders [81]. An important role for Lyn in both chronic and
Proliferative disorders [81]. An important role for Lyn in both chronic and acute myeloid leukaemic cells has been suggested by several studies [75,76,82-84]. In an analysis of primary AML cells, 76 had elevated Lyn kinase activity, while none had constitutive JAK2 activation; moreover, inhibition of Lyn OrnipressinMedChemExpress POR-8 activity (using genetic and small molecule inhibitors) in AML cell lines substantially decreased cell growth [75]. Recent studies have also confirmed the common activation of Lyn in primary AML, and its critical role in maintaining proliferation and anti-apoptotic pathways in these cells [78]. Further, Lyn is a signalingcomponent of the Fms-like tyrosine kinase 3/internal tandem duplication (FLT3/ITD)-specific pathway linking FLT3/ITD to STAT5. FLT3/ITD is the most common mutation in human adult AML and Lyn binds with high affinity to this mutated receptor. Down-regulation of Lyn/Src family kinases in these AML cells by siRNA or small molecule inhibitors substantially ameliorated their growth in vitro and tumor establishment and growth in vivo, as well as phosphorylation of important downstream FLT3/IDT mediators such as STAT5 [85]. One of the standard treatments for AML is through differentiation induction by all-trans-retinoic acid (ATRA), and recent data has shown a potentially important modulation of this pathway by Lyn [86]. Here, Lyn inhibitors, such as Dasatinib, enhanced the effectiveness of ATRA through a positive feedback loop involving a scaffolding protein (KSR1) that complexes regulators of the MAP kinase pathway with Lyn, illustrating the potential for using Lyn/SFK inhibitors as adjunct therapies in combination with other anti-AML effectors [86]. A number of reports have illustrated that while the BCR-Abl fusion protein is the initiating molecule for CML, there is a crucial down-stream role for Lyn PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28388412 in BCRAbl induced leukemogenesis [82,83]. Not only does Lyn bind BCR-Abl and is activated by it [84], but Lyn can also phosphorylate BCR-Abl and modulate its ability to transform cells [87]. There are other direct links between Lyn and BCR-Abl signaling pathways as Lyn phosphorylates the Y177 motif of BCR-Abl [88], resulting in recruitment of the adaptor Gab2, a principle activator of the PI-3 kinase pathway, both of which are essential for BCR-Abl oncogenesis [89]. Lyn is also involved in other signaling pathways in CML cells, including through BCR-Abl activation of JAK2 that then activates Lyn by preventing SHP1 from turning off Lyn activity [90]. Interestingly, when the CML cell line K562 is selected for Imatinib resistance, the resultant cell line (K562R) has elevated Lyn levels and kinase activity, as apposed to BCR-Abl mutations; their sensitivity to this drug is regained upon down-regulation of Lyn [76]. Significantly, in primary CML cells from patients that have acquired Imatinib resistance, elevated Lyn levels also appear commensurate with the development of this drug resistance [76]. A study of drugresistant blast crisis BCR-Abl (+) leukaemic cells showed that Lyn is crucial for their survival. Ablation of Lyn from these cells resulted in the induction of apoptosis, while normal CD34+ stem cells were not affected by Lyn ablation [91]. Detailed phosphoproteomic analysis of Bcr-Abl transformed cells [92] has revealed significant cross talk between Bcr-Abl and the negative feedback loops that control Lyn signaling via Cbp/PAG1. The protein Cbp/ PAG1 [15,17,18] is a scaffold molecule involved in recruiting both the inhib.
