The Cu(I) centre, it was was Given that the chlorate ion is fundamental to the oxidation from the Cu(I) centre, it theorised that that a peak in chloride would occur alongside the Cu elution peak, andso this was theorised a peak in chloride would take place alongside the Cu elution peak, and so this was analysed for by IC, at the same time as MNITMT Epigenetics sulphate concentrations. The concentrations of Cl- and SO4 2- by IC, at the same time as sulphate concentrations. The concentrations of Cl- and analysed 2- are presented alongside the Cu elution profile in Figure 13. A peak in chloride conSO4 are presented alongside the Cu elution profile in Figure 13. A peak in chloride concentration centration was observed simultaneously with peak Cu elution,maximum concentration of 0.02 was observed simultaneously with peak Cu elution, having a with a maximum concentration of – (714 mg/L). A substantial peak in sulphate concentration (maximum 0.085 M SO4 2- M Cl 0.02 M Cl- (714 mg/L). A substantial peak in sulphate concentration (maximum 0.085(5646 mg/L)) was also was also detected, occurring before in Cu andin Cu and Cl, the M SO42- (5646 mg/L)) detected, occurring prior to the peak the peak Cl, and before and before the increased pH. period of period of improved pH. The in Cl- is not unexpected, and along with the parallel occurrence of Cu Cl- Cl- elution The peakpeak in Cl- just isn’t unexpected,the parallel occurrence of Cu and and elution confirmed that the technique of Cu liberation is a redox interaction among ClO3- and Cu(I), confirmed that the technique of Cu liberation is a redox interaction among ClO3- and Cu(I), whereby ClO3- is Fmoc-Gly-Gly-OH ADC Linkers decreased and Cu(I) Cu(I) oxidised to Cu(II). Nevertheless, the spike in whereby ClO3- is reduced to Cl-to Cl- and oxidised to Cu(II). However, the largelarge spike in sulphate concentration is specifically substantial provided that sulphate was introduced sulphate concentration is specifically significant provided that no no sulphate was introduced to the technique during the elution cycle. Even though to the system during the elution cycle. Although the PLS employed for loading did include sulphate, a PLS utilized for loading did include sulthorough rinse cycle applying 18 18 M deionised water was performed priorto elution such M deionised water was performed before elution phate, a thorough rinse cycle using such that residual sulphate concentration was below 0.002 M prior to the start out with the sulphate that residual sulphate concentration the sulpeak in Figure 13. phate peak in Figure 13.pHEng 2021, two Eng 2021, 2,527Figure 13. Concentrations of Cu, Cl and SO42- 4 two effluent options throughout elution of S914 S914 at Figure 13. Concentrations of Cu, Cl-, – , and SOin – in effluent options during elution ofat two BV/h working with 0.5 M NaClO3 (pH 1.95, HCl media, identical identical Cu elution as presented in Figure 12). 2 BV/h employing 0.5 M NaClO3 (pH 1.95, HCl media,Cu elution profileprofile as presented in Figure 12).It’s essential to note that anion concentrations presented in Figure 13 have already been It is important to note that anion concentrations presented in Figure 13 happen to be corrected to think about the concentrations in each the calibration blank and eluent remedy, corrected to think about the concentrations in both the calibration blank and eluent solution, such that the presented information represents only extra anions introduced towards the technique. such that the presented data represents only further anions introduced for the technique. Consequently, the sulphate peak detected in effluent options could have only or.
