E to efficiently switch the Cre-LoxP reporter system in injected embryos, resulting inside a massive variation of fluorescent cells distributed inside the full zebrafish in a mosaic pattern. In contrast, injected EVs derived from cells with high Cre expression were able to colour switch cells in only 1 out of 60 injected zebrafish. The low efficiency in EV-mediated Creprotein or RNA transfer is correlated with tiny quantity of Cre-mRNA present inside the four nL EV isolate that contained approximately 30 10-14 pg in comparison with the 50 pg present inside the four nL synthetic Cre-mRNA answer. Summary/Conclusion: The Zebrabow Cre-LoxP reporter method is an efficient reporter for Cre activity and could for that reason be a perfect model technique to study EV-transfer in vivo. Even so, the amount of EVmediated transfer of Cre-mRNA is too low having a single injection of 4 nL of purified EVs from Cre-expressing cell lines. This extremely low efficacy can nicely be explained by the relative low Cre-mRNA quantity in EVs as well as the small volume that will maximally be injected inside the yolk of zebrafish embryos.OF11.Pre-metastatic cancer exosomes induce immune surveillance by patrolling monocytes Michael P. Plebanek1; Nicholas Angeloni1; Elena Vinokour1; Anna Henkin2; Dalia Martinez-Marin3; Stephanie Filleur3; Reshma Bhowmick4; Jack Henkin5; Stephen Miller1; Igal Ifergan1; Yesung Lee6; Iman Osman6; Shad Thaxton1; Olga Volpert7 Northwestern University, Chicago, IL, USA; 2Massachusetts Institute of Technologies, Boston, MA, USA; 3Texas Tech University, Lubbock, TX, USA; 4 University of Texas MD Anderson Cancer Center, Houston, TX, USA; five Northwestern University, Evanston, IL, USA; 6New York University, New York, NY, USA; 7MD Anderson Cancer Center, Houston, TX, USAOF11.Zebrabow as in vivo model technique to monitor vesicles mediated transfer in cancer Martin E. van Royen1; Wilma Teubel2; Thomas A. Hartjes3; Tjakko van Ham4; Guido W. Jenster1 Department of Pathology, Erasmus Optical Imaging Centre, Erasmus MC, Rotterdam, The Netherlands; 2Department of Urology, Erasmus MC, Rotterdam, The Netherlands; 3Erasmus Health-related Center, Rotterdam, The Netherlands; 4Department of Clinical genetics, Erasmus MC, Rotterdam, The NetherlandsBackground: Cancer exosomes are frequently involved inside the suppression of innate immune responses. Monocytes and macrophages are essential within the metastatic microenvironments, in tumour-promoting or tumour-suppressive capacities. Non-classical or patrolling Ly6C low monocytes (PMo) were identified for the ability to remove broken cells and rely on nuclear receptor Nr4a1 for ABL1 Proteins custom synthesis survival. Not too long ago, Nr4a1-positive PMo had been implicated in scavenging metastatic tumour cells within the lungs. On the other hand, the events that manage PMo in the metastatic niche remain unknown.ISEV 2018 abstract bookMethods: We isolated and tested exosomes from spontaneously occurring and artificially generated metastatic/ non-metastatic melanoma cells and tested them in vivo for altering metastatic capacity of human and mouse cells. The effect on bone marrow Serpin (Protease Inhibitor) Proteins Biological Activity myeloid cells was examined by FACS and dependence on certain cell sorts was determined working with clodronate liposomes and neutralizing antibodies. The effects on macrophages were examined in functional and biochemical assays. The relevance of the findings was assessed by a functional and biomarker evaluation of patient exosomes. Benefits: Exosomes from non-metastatic melanoma cells (ExoNM) are taken up by myeloid cells within the bone marrow and result in an expansion of Ly6C low mo.
