Esentative individuals of a minimum of 50 seedlings in three or extra replicate experiments. Various letters above the bars indicate significantly diverse imply values (p 0.05) primarily based on Duncan’s several variety test., Figure S3: Effect of sound wave treatments around the root growth of Arabidopsis wild-type (Col-0) below auxin transport inhibitor naphthylphthalamic acid (NPA) situations. (A) 20 and (B) 50 NPA. An optical microscope was employed to observe root development (0) and root recommendations (0). Scale bars represent 1 mm (Top rated) and 200 (Bottom). (C) Main root and root tip length measurement for each and every treatment ( tandard error). Different letters indicate substantially distinctive mean values (p 0.05) primarily based on Duncan’s various variety test. This experiment was conducted in triplicate, and every experiment measured 250 seedlings., Figure S4: Impact of sound wave remedies on root development of wild-type (Col-0) and pin3 pin4 pin7 triple mutant Arabidopsis thaliana. (A) An optical microscope was utilised to observe root development (0) and root guidelines (0). Scale bars represent two mm (A) and 200 (B). (C) Principal root and root tip length measurement for every CXCR4 Antagonist list single remedy ( tandard error). Distinctive letters indicate significantly distinct imply values (p 0.05) primarily based on Duncan’s various range test. This experiment was performed in triplicate, and every experiment measured 250 seedlings, Figure S5: Effect of sound wave treatments around the root development of Arabidopsis wildtype (Col-0) under auxin biosynthesis inhibitor (yucasin) conditions. (A) 20 and (B) 50 yucasin. An optical microscope was used to observe root development (0) and root guidelines (0). Scale bars represent 1 mm (Prime) and 200 (Bottom). (C) Principal root and root tip length measurement for each and every therapy ( tandard error). Diverse letters indicate substantially different imply values (p 0.05) primarily based on Duncan’s many variety test. This experiment was carried out in triplicate, and each and every experiment measured 250 seedlings., Figure S6: Effect of sound wave treatment options on root growth of wild-type (Col-0), and ahk3-1 and ahk4 mutant Arabidopsis thaliana. (A) An optical microscope was used to observe root development (0) and root guidelines (0). Scale bars represent two mm (A) and 200 (B). (C) Principal root and root tip length measurement for each and every remedy ( tandard error). Different letters indicate substantially unique mean values (p 0.05) based on Duncan’s various range test. This experiment was performed in triplicate, and every single experiment measured 250 seedlings., Figure S7: Effect of sound wave therapies on root growth of wild-type (Col-0), tir1-1 single mutant, and tir1-1 afb2-3 double mutant Arabidopsis thaliana. (A) An optical microscope was applied to observe the development of roots (ten) and root recommendations (0). Scale bars represent two mm (A)Int. J. Mol. Sci. 2021, 22,14 ofand 200 (B). (C) Key root and root tip length measurement for every single treatment ( tandard error). Distinctive letters indicate significantly various mean values (p 0.05) based on Duncan’s many variety test. This experiment was conducted in triplicate, and each experiment measured 250 seedlings, Table S1: qRT-PCR primer sequences employed within this study. Author Bcl-2 Inhibitor Synonyms Contributions: Conceptualization, M.-J.J. and J.Y.K.; formal evaluation, M.-J.J. and J.Y.K.; investigation, J.Y.K. and J.A.K.; data curation, H.-J.L.; writing–original draft preparation, J.Y.K. and H.-J.L.; writing–review and editing, M.-J.J. and H.-J.L.; visualization, J.Y.K.; funding acquisition, M.-J.J. All.
