Esentative individuals of at the least 50 seedlings in three or much more replicate experiments. Distinctive letters above the bars indicate substantially different imply values (p 0.05) primarily based on Duncan’s various range test., Figure S3: Impact of sound wave therapies around the root development of Arabidopsis COX-1 Inhibitor manufacturer wild-type (Col-0) under auxin transport inhibitor naphthylphthalamic acid (NPA) conditions. (A) 20 and (B) 50 NPA. An optical microscope was utilised to observe root growth (0) and root ideas (0). Scale bars represent 1 mm (Top) and 200 (Bottom). (C) Major root and root tip length measurement for every single treatment ( tandard error). Distinctive letters indicate drastically unique mean values (p 0.05) primarily based on Duncan’s several range test. This IP Activator custom synthesis experiment was conducted in triplicate, and every experiment measured 250 seedlings., Figure S4: Effect of sound wave treatment options on root development of wild-type (Col-0) and pin3 pin4 pin7 triple mutant Arabidopsis thaliana. (A) An optical microscope was made use of to observe root growth (0) and root ideas (0). Scale bars represent two mm (A) and 200 (B). (C) Primary root and root tip length measurement for each and every treatment ( tandard error). Diverse letters indicate significantly various imply values (p 0.05) primarily based on Duncan’s a number of range test. This experiment was conducted in triplicate, and each experiment measured 250 seedlings, Figure S5: Effect of sound wave treatments around the root development of Arabidopsis wildtype (Col-0) beneath auxin biosynthesis inhibitor (yucasin) circumstances. (A) 20 and (B) 50 yucasin. An optical microscope was made use of to observe root development (0) and root tips (0). Scale bars represent 1 mm (Top) and 200 (Bottom). (C) Primary root and root tip length measurement for every single therapy ( tandard error). Different letters indicate substantially various mean values (p 0.05) based on Duncan’s several range test. This experiment was conducted in triplicate, and every experiment measured 250 seedlings., Figure S6: Impact of sound wave treatments on root growth of wild-type (Col-0), and ahk3-1 and ahk4 mutant Arabidopsis thaliana. (A) An optical microscope was made use of to observe root development (0) and root suggestions (0). Scale bars represent two mm (A) and 200 (B). (C) Key root and root tip length measurement for each remedy ( tandard error). Different letters indicate drastically distinctive mean values (p 0.05) based on Duncan’s multiple range test. This experiment was performed in triplicate, and every experiment measured 250 seedlings., Figure S7: Impact of sound wave treatments on root development of wild-type (Col-0), tir1-1 single mutant, and tir1-1 afb2-3 double mutant Arabidopsis thaliana. (A) An optical microscope was applied to observe the development of roots (ten) and root strategies (0). Scale bars represent 2 mm (A)Int. J. Mol. Sci. 2021, 22,14 ofand 200 (B). (C) Principal root and root tip length measurement for each and every remedy ( tandard error). Different letters indicate drastically diverse imply values (p 0.05) based on Duncan’s a number of variety test. This experiment was carried out in triplicate, and each and every experiment measured 250 seedlings, Table S1: qRT-PCR primer sequences utilized in this study. Author Contributions: Conceptualization, M.-J.J. and J.Y.K.; formal analysis, M.-J.J. and J.Y.K.; investigation, J.Y.K. and J.A.K.; data curation, H.-J.L.; writing–original draft preparation, J.Y.K. and H.-J.L.; writing–review and editing, M.-J.J. and H.-J.L.; visualization, J.Y.K.; funding acquisition, M.-J.J. All.
