nrelated to Bt-resistance coding genes (FigureBt-resistance linked gene was MAP3K5/ASK1 custom synthesis significantly less than one hundred kb up- or downstream from the lncRNA (LTE4 web Figure 4A ). The proximal CYP was 7.868 kb from lncRNA LOC11350610 (this lncRNA was upregulated) (Figure 4A), the proximal ABC transporter 50.672 kb from lncRNA LOC110369725 (this lncRNA was downregulated) (Figure 4B), along with the serine protease 0.646 kb from lncRNA LOC110382674 (this lncRNA was located only inside the resistant strain) (Figure 4C). The lncRNA presented in Figure 4D was downregulated, and also the lncRNA presented in Figure 4E was located only inside the susceptible strain.Insects 2022, 13,(Figure 4E). Each proximal Bt-resistance linked gene was significantly less than one hundred kb up- or downstream from the lncRNA (Figure 4A ). The proximal CYP was 7.868 kb from lncRNA LOC11350610 (this lncRNA was upregulated) (Figure 4A), the proximal ABC transporter 50.672 kb from lncRNA LOC110369725 downstream was downregulated) resistance linked gene was much less than 100 kb up- or (this lncRNAfrom the lncRNA (Fig(Figure 4B), and the serine protease 0.646 from lncRNA LOC11350610 (this lncRNA was ure 4A ). The proximal CYP was 7.868 kbkb from lncRNA LOC110382674 (this lncRNA of 18 was located only in the resistant proximal ABC transporter 50.672 kb in Figure9 4D upregulated) (Figure 4A), the strain) (Figure 4C). The lncRNA presentedfrom lncRNA was downregulated,lncRNA was downregulated)in Figure4B), and the serine protease plus the lncRNA presented (Figure 4E was found only in the LOC110369725 (this susceptible strain. 0.646 kb from lncRNA LOC110382674 (this lncRNA was found only inside the resistant strain) (Figure 4C). The lncRNA presented in Figure 4D was downregulated, along with the lncRNA presented in Figure 4E was located only in the susceptible strain.Figure 3. Workflow for identifying statistically differentiated lncRNAs coding genes in toto and Figure three. Workflow for identifying statistically in Bt-resistance lncRNAs proximal in toto and those Figure with functions recognized to have a function differentiated lncRNAsare coding genesstatistically those 3. Workflow for identifying statistically differentiated that coding genes to in toto and with with functions known to possess ain Bt-resistance that happen to be proximal size to statistically differendifferentiated known to have a function role in Bt-resistance which can be proximal on the scaffolds, even these functions lncRNAs. Proximity measurements have been limited by theto statistically differentiated lncRNAs. Proximity measurements million base by thecis and the scaffolds,scaffolds, although although proximity is defined as 1 have been limited pairs by of size from despite the fact that proximity tiated lncRNAs. Proximity measurements had been limitedsize the trans on the the lncRNA. For every proximal as 1 million and lncRNA, a BLASTn alignment was lncRNA. For every coding gene and proximity is defined as base pairsbase pairs cis and trans lncRNA. also every single proximalproximal coding is defined coding gene 1 million cis and trans in the in the For performed to assess potential pseudogenes. gene and lncRNA, a alignment was also conducted to assess potential potential pseudogenes. lncRNA, a BLASTn BLASTn alignment was also conducted to assess pseudogenes.(A)Figure four. Cont.Insects 2022, 13, 12 Insects 2022, 1, x10 of 18 10 of(B)(C)Figure four. Cont.Insects 2022, 13, 12 Insects 2022, 1, x11 of 18 11 of(D)(E)Figure 4. Genomic scaffold for lncRNAs and identification of proximal protein-coding genes. The Figure four. Genomic scaffold for lncRNAs and id
