nrelated to Bt-resistance coding genes (FigureBt-resistance associated gene was less than one hundred kb up- or downstream from the lncRNA (Figure 4A ). The proximal CYP was 7.868 kb from lncRNA LOC11350610 (this lncRNA was upregulated) (Figure 4A), the proximal ABC transporter 50.672 kb from lncRNA LOC110369725 (this lncRNA was downregulated) (Figure 4B), and the serine protease 0.646 kb from lncRNA LOC110382674 (this lncRNA was discovered only within the resistant strain) (Figure 4C). The lncRNA presented in Figure 4D was HD1 drug downregulated, as well as the lncRNA presented in Figure 4E was discovered only inside the susceptible strain.Insects 2022, 13,(Figure 4E). Each proximal Bt-resistance linked gene was much less than 100 kb up- or downstream from the lncRNA (Figure 4A ). The proximal CYP was 7.868 kb from lncRNA LOC11350610 (this lncRNA was upregulated) (Figure 4A), the proximal ABC transporter 50.672 kb from lncRNA LOC110369725 downstream was downregulated) resistance connected gene was less than 100 kb up- or (this lncRNAfrom the lncRNA (Fig(Figure 4B), along with the serine protease 0.646 from lncRNA LOC11350610 (this lncRNA was ure 4A ). The proximal CYP was 7.868 kbkb from lncRNA LOC110382674 (this lncRNA of 18 was identified only in the resistant proximal ABC transporter 50.672 kb in Figure9 4D upregulated) (Figure 4A), the strain) (Figure 4C). The lncRNA presentedfrom lncRNA was downregulated,lncRNA was downregulated)in Figure4B), as well as the serine protease plus the lncRNA presented (Figure 4E was located only within the LOC110369725 (this susceptible strain. 0.646 kb from lncRNA LOC110382674 (this lncRNA was identified only inside the resistant strain) (Figure 4C). The lncRNA presented in Figure 4D was downregulated, as well as the lncRNA presented in Figure 4E was located only within the susceptible strain.Figure three. Workflow for identifying statistically differentiated lncRNAs coding genes in toto and Figure 3. Workflow for identifying statistically in Bt-resistance lncRNAs proximal in toto and these Figure with functions known to have a role differentiated lncRNAsare coding genesstatistically these 3. Workflow for identifying statistically differentiated that coding genes to in toto and with with functions identified to have ain Bt-resistance which might be proximal size to statistically differendifferentiated identified to possess a part role in Bt-resistance which can be proximal with the scaffolds, even these functions lncRNAs. Proximity KDM4 Storage & Stability measurements have been limited by theto statistically differentiated lncRNAs. Proximity measurements million base by thecis and also the scaffolds,scaffolds, despite the fact that even though proximity is defined as 1 had been restricted pairs by of size from despite the fact that proximity tiated lncRNAs. Proximity measurements were limitedsize the trans of your the lncRNA. For each and every proximal as 1 million and lncRNA, a BLASTn alignment was lncRNA. For every coding gene and proximity is defined as base pairsbase pairs cis and trans lncRNA. also every proximalproximal coding is defined coding gene 1 million cis and trans from the in the For conducted to assess possible pseudogenes. gene and lncRNA, a alignment was also performed to assess prospective possible pseudogenes. lncRNA, a BLASTn BLASTn alignment was also performed to assess pseudogenes.(A)Figure four. Cont.Insects 2022, 13, 12 Insects 2022, 1, x10 of 18 10 of(B)(C)Figure four. Cont.Insects 2022, 13, 12 Insects 2022, 1, x11 of 18 11 of(D)(E)Figure 4. Genomic scaffold for lncRNAs and identification of proximal protein-coding genes. The Figure four. Genomic scaffold for lncRNAs and id
