ion have been were considerably changed (log2 2). Among them, the Expression levels of GA synthesis connected genes were sigDP Inhibitor Purity & Documentation nificantly decreased, including GA20ox (Traes1A01G263600 and Traes3D01G401400), which encoded the crucial enzymes on the GA synthetic pathway, and terpene synthetase gene (TraesCS7A01G002400) was decreased about two.1, four.2, and 14.5 occasions asInt. J. Mol. Sci. 2021, 22, x FOR PEER REVIEW6 ofInt. J. Mol. Sci. 2021, 22,IL-2 Modulator custom synthesis substantially changed (log2 two). Among them, the expression levels of GA synthesis six of 12 associated genes have been considerably decreased, which include GA20ox (Traes1A01G263600 and Traes3D01G401400), which encoded the key enzymes with the GA synthetic pathway, and terpene synthetase gene (TraesCS7A01G002400) was decreased about 2.1, 4.2, and 14.5 occasions as compared with WT (Figure 4A), respectively. On the other hand, the of GA2ox compared with WT (Figure 4A), respectively. Alternatively, the expressionexpression of GA2ox (TraesCS3B01G166100) and gene (TraesCS6D01G166900, TraesCS6B01G074100 (TraesCS3B01G166100) and cytochrome P450cytochrome P450 gene (TraesCS6D01G166900, and TraesCS5D01G323800) TraesCS5D01G323800) which related to GA inactivation,DELLA TraesCS6B01G074100 and which connected to GA inactivation, and also the expression of plus the (TraesCS1A01G413800 and TraesCS3B01G262400), the essential unfavorable the crucial expression of DELLA (TraesCS1A01G413800 and TraesCS3B01G262400), regulators of GA signal transduction pathway,transduction pathway, were substantially increased with adverse regulators of GA signal had been considerably increased with 1.four, 1.6, 1.5, 1.three, 1.four and 1.6, 1.5, 1.3, 1.four and 1.4 instances of WT,than that of WT, respectively. Furthermore, it was 1.4, 1.four times higher than that higher respectively. In addition, it was discovered that the expression levelsexpression levels of GA signal transduction genes, and discovered that the of GA signal transduction genes, TPR (TraesCS6B01G005400 TPR TraesCS4A01G244800) wereTraesCS4A01G244800)times decreased and two.two instances (p(p 0.01) (TraesCS6B01G005400 and decreased about 3.three have been (p 0.01) about three.3 times 0.01) compared with(p 0.01) outcomes indicated thatThe benefits indicated that TaWUS-like and and two.2 times WT. The compared with WT. TaWUS-like might inhibit the synthesis may well functiontheGA, which and function of GA, which was consistent using the phenotype of inhibit of synthesis was constant with the phenotype of dwarfing plants with a decrease GA content in internodes. dwarfing plants having a reduced GA content material in internodes.Figure four. Expression levels the downstream GA-, BR- (A) and cell division-related genes (B) regulated by by TaWUS-likeFigure 4. Expression levels ofof the downstream GA-, BR- (A) and cell division-related genes (B) regulatedTaWUS-like-OE. OE. Red and green circles indicate the expression from the genes was changed upward and downward, respectively. Red and green circles indicate the expression in the genes was changed upward and downward, respectively.In addition, the expression levels of genes involved in the BR pathway in which five Furthermore, the expression levels of genes involved within the BR pathway in which 5 genesrelated to synthesis and 3 related to signal transduction were also changed siggenes related to synthesis and 3 associated to signal transduction had been also changed drastically (log2 The The two within the within the BR signal have been significantly changed, nificantly (log2 2). two). two genes genes BR signal pathway pathway had been significantly changed, se
