Active Recombinant Human CD47 protein, His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Monoclonal Anti-Human CD47 Antibody, Human IgG4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, His,Avitag with a linear range of 0.1-3 ng/mL.Immobilized Human SIRP alpha, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, His,Avitag with a linear range of 0.2-25 ng/mL. | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 16.9 kDa. The protein migrates as 32-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Gln19-Pro139 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.