Active Recombinant Human CD97 protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD97 at 10 μg/ml (100 μl/well) can bind recombinant human CD55 at a linear range of 0.46-30 μg/ml.2. Using the Octet RED System, the affinity constant (Kd) of Anti-CD97 Antibody bound to CD97 Protein, Human, Recombinant (His Tag) was 0.2 nM. | Molecular Mass : The recombinant human CD97 consists of 389 amino acids and predictes a molecular mass of 42.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD97 is approximately 60-70 kDa due to glycosylation. | Protein Length : Met1-Gln398 | Endotoxin : | Purity : > 85 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.