Active Recombinant Human CEACAM1 protein(Met1-Gly428), His&hFc-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : C-His&hFc | Protein length : Met1-Gly428 | Form : Lyophilized from sterile 100 mM Glycine, 500 mM NaCl, 5 % Glycerol, 50 mM Arg Hcl, 50 mM Glu, 0.01 % tween 20, pH 5.0. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized CEACAM8-His at 10 μg/mL (100 μL/well) can bind CEACAM1-Fch, the EC50 of CEACAM1-Fch is 70-200 ng/mL. | Molecular Mass : The secreted recombinant human CEACAM1/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 642 amino acids and predicts a molecular mass of 71.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCEACAM1 is approximately 124.8 kDa in SDS-PAGE under reducing conditions. | Endotoxin : | Purity : > 90 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.