Active Recombinant Human CD86 Protein, His & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His & Avi | Predicted N Terminal : Leu 26 | Form : Lyophilized from sterile PBS, pH7.4, 10% trehalose. | Bio-activity : Immobilized Human CTLA-4, Fc Tag at 10μg/mL (100 μL/well) can bind Biotinylated Human B7-2, His Tag with a linear range of 0.01-0.15 μg/mL. | Molecular Mass : Recombinant Human CD86 Protein has a calculated MW of 28 kDa. The protein migrates as 45-65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. | Protein length : Leu 26 – Pro 247 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >90% as determined by SDS-PAGE. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.