Recombinant Human CD86 protein, His & T7-tagged

Specification :
| Description : This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. | Source : E. coli | Species : Human | Tag : His & T7 | Form : Freeze-dried powder | Molecular Mass : Predicted Molecular Mass: 30.9kDa | Protein length : Gln46~Cys282 (Accession # P42081) | Endotoxin : | Purity : >95% | Characteristic : The isoelectric point is 8.2. | Applications : SDS-PAGE; WB; ELISA; IP | Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. | Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. | Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl | Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4