Recombinant Human CDH2 Protein, His-tagged

Specification :
| Description : Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-Cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical Cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the “cadherin switch.” N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers. | Source : E. coli | Species : Human | Tag : His | Molecular Mass : ~62 kDa | AA Sequence : DWVIPPINLPENSRGPFPQELVRIR SDRDKNLSLRYSVTGPGADQPPTGI FIINPISGQLSVTKPLDREQIARFH LRAHAVDINGNQVENPIDIVINVID MNDNRPEFLHQVWNGTVPEGSKPGT YVMTVTAIDADDPNALNGMLRYRIV SQAPSTPSPNMFTINNETGDIITVA AGLDREKVQQYTLIIQATDMEGNPT YGLSNTATAVITVTDVNDNPPEFTA MTFYGEVPENRVDIIVANLTVTDKD QPHTPAWNAVYRISGGDPTGRFAIQ TDPNSNDGLVTVVKPIDFETNRMFV LTVAAENQVPLAKGIQHPPQSTATV SVTVIDVNENPYFAPNPKIIRQEEG LHAGTMLTTFTAQDPDRYMQQNIRY TKLSDPANWLKIDPVNGQITTIAVL DRESPNVKNNIYNATFLASDNGIPP MSGTGTLQIYLLDINDNAPQVLPQE AETCETPDPNSINITALDYDIDPNA GPFAFDLPLSPVTIKRNWTITRLNG DFAQLNLKIKFLEAGIYEVPIIITD SGNPPKSNISILRVKVCQCDSNGDC TDVDRIVGAGLGTGA | Purity : Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE). | Notes : For research use only, not for use in diagnostic procedure. | Storage : Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles. | Concentration : ≥0.5 mg/mL | Storage Buffer : PBS, 4M Urea, pH7.4