Active Recombinant Human CDK7 Protein, GST-Tagged

Specification :
| Description : The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008] | Source : Insect Cell | Species : Human | Tag : GST | Form : Liquid | Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CTD3 peptide. ATP: 100 μM. | Molecular Mass : 66 kDa | Purity : 99 % by SDS-PAGE/CBB staining | Applications : Functional StudySDS-PAGE | Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. | Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)