Active Recombinant Human CTLA4 protein, mFc-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : mFc-Avi | Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Human B7-1, Fc Tag (HPLC-verified) at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag with a linear range of 0.3-5 ng/mL.Immobilized Human B7-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag with a linear range of 0.3-2 ng/mL.Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag with a linear range of 0.2-2 ng/mL. | Molecular Mass : This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 42.1 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Ala37-Phe162 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.