Acitve Recombinant Human SIRPA protein(Met1-Arg369(S44L, S50T, I52T, H54R, V57A)), mFc-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : C-mFc | Protein length : Met1-Arg369(S44L, S50T, I52T, H54R, V57A) | Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human CD47-His at 2 μg/ml (100 μl/well) can bind Human SIRPa(V8)-mFc, the EC50 of Human SIRPa(V8)-mFc is 2-14 ng/mL. | Molecular Mass : The recombinant Human SIRP alphaV8 consists of 572 amino acids and predicts a molecular mass of 63.6 kDa. As a result of glycosylation, it migRates as an approximately 80.4 kDa band in SDS-PAGE under reducing conditions. | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.