Active Recombinant Human CSF1 protein, His-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. | Bio-activity : Immobilized Human M-CSF R, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag with a linear range of 0.1-2 ng/mL. Immobilized Biotinylated Human M-CSF, His,Avitag at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag with a linear range of 1-39 ng/mL. | Molecular Mass : This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 28.8 kDa. The protein migrates as 35-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein Length : Glu 33 – Arg 255 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >90% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.