Recombinant Human CTLA4, Fc Chimera

Specification :
| Cat. No. : CTLA4-1913H | Description : CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. | Source : E.Coli. | Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val. | Form : Purified. | Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. | Dimers & Aggregates : | Endotoxin : | Protein Content : Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of CTLA-4 as a Reference Standard. | Application : 1) Molecular standard (Western, ELISA) in studying secreted BMP-4. 2) Preparing antibodies for BMP-4 monomer. 3) Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE. | Biological Activity : rHuCTLA-4 is fully biologically active when compared to standards. | Storage : -20°C, avoid freezing and thawing cycles after reconstitution.