Active Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human / Cynomolgus / Rhesus Monkey | Tag : Fc & Avi | Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Human B7-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.15-5 μg/mL (QC tested).Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 2.4-39 ng/mL (Routinely tested).Immobilized Human B7-1, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.313-5 μg/mL (Routinely tested). | Molecular Mass : 43.6 kDa | Protein length : Asn 19 – Pro 152 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Notes : Please avoid repeated freeze-thaw cycles. | Stability : No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution. | Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.