Active Recombinant Human ENTPD1 Protein, His-Avi-tagged, Biotinylated

Specification :
| Description : CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells(Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities. | Source : HEK293 | Species : Human | Tag : His-Avi | Form : Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant. | Bio-activity : Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 3,000 pmol/min/μg. | Molecular Mass : The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Protein length : Thr 38 – Val 478 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : Please avoid repeated freeze-thaw cycles.This product is stable after storage at:The product MUST be stored at -70°C or lower upon receipt;-70°C for 3 months under sterile conditions. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.