Active Recombinant Human EPCAM Protein, Fc-tagged, Alexa Fluor 555 conjugated

Specification :
| Source : Mammalian cells | Species : Human | Tag : Fc | Form : Lyophilized | Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.When 5 × 10^4 cells/well are added to recombinant human EpCAM/Fc Chimera and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is typically 0.7-2.8 μg/mL. Measured by its binding ability in a functional ELISA. The activity of this protein is measured in a homophilic binding assay. | Molecular Mass : Predicted Molecular Mass: 54 (monomer) kDa; SDS-PAGE: 60-70 kDa, reducing conditions | Protein length : Gln24-Lys265 | N-terminal Sequence Analysis : Gln 24 | Endotoxin : | Purity : > 90 % by SDS-PAGE and analyzed by silver stain | Characteristic : Disulfide-linked homodimerLabeled with Alexa Fluor 555 via aminesWith an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. | Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. | Concentration : 100 μg/mL | Storage Buffer : Lyophilized from a 0.2 μm filtered solution in PBS. | Reconstitution : Reconstitute at 100 μg/mL in sterile PBS. | Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.