Recombinant Human DGKG Protein, GST-tagged

Specification :
| Description : This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] | Source : Wheat Germ | Species : Human | Tag : GST | Molecular Mass : 112.7 kDa | AA Sequence : MGEERWVSLTPEEFDQLQKYSEYSS KKIKDALTEFNEGGSLKQYDPHEPI SYDVFKLFMRAYLEVDLPQPLSTHL FLAFSQKPRHETSDHPTEGASNSEA NSADTNIQNADNATKADEACAPDTE SNMAEKQAPAEDQVAATPLEPPVPR SSSSESPVVYLKDVVCYLSLLETGR PQDKLEFMFRLYDSDENGLLDQAEM DCIVNQMLHIAQYLEWDPTELRPIL KEMLQGMDYDRDGFVSLQEWVHGGM TTIPLLVLLGMDDSGSKGDGRHAWT MKHFKKPTYCNFCHIMLMGVRKQGL CCTYCKYTVHERCVSRNIPGCVKTY SKAKRSGEVMQHAWVEGNSSVKCDR CHKSIKCYQSVTARHCVWCRMTFHR KCELSTLCDGGELRDHILLPTSICP ITRDRPGEKSDGCVSAKGELVMQYK IIPTPGTHPLLVLVNPKSGGRQGER LNFFRDTPDFRVLACGGDGTVGWIL DCIDKANFAKHPPVAVLPLGTGNDL ARCLRWGGGYEGGSLTKILKDIEQS PLVMLDRWHLEVIPREEVENGDQVP YSIMNNYFSIGVDASIAHRFHVMRE KHPEKFNSRMKNKLWYFEFGTSETF AATCKKLHDHIELECDGVGVDLSNI FLEGIAILNIPSMYGGTNLWGENKK NRAVIRESRKGVTDPKELKFCVQDL SDQLLEVVGLEGAMEMGQIYTGLKS AGRRLAQCASVTIRTNKLLPMQVDG EPWMQPCCTIKITHKNQAPMMMGPP QKSSFFSLRRKSRSKD | Applications : Enzyme-linked Immunoabsorbent AssayWestern Blot (Recombinant protein)Antibody ProductionProtein Array | Notes : Best use within three months from the date of receipt of this protein. | Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. | Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.