Active Recombinant Human DKK1 protein, Fc-Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : Fc-Avi | Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Biotinylated Human Dkk-1, Fc,Avitag at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.5-16 ng/mL. | Molecular Mass : This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Thr32-His266 | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.