Recombinant Human DICER1 Protein, Myc/DDK-tagged, C13 and N15-labeled

Specification :
| Description : This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. | Source : HEK293 | Species : Human | Tag : Myc/DDK | Molecular Mass : 218.5 kDa | AA Sequence : MKSPALQPLSMAGLQLMTPASSPMG PFFGLPWQQEAIHDNIYTPRKYQVE LLEAALDHNTIVCLNTGSGKTFIAV LLTKELSYQIRGDFSRNGKRTVFLV NSANQVAQQVSAVRTHSDLKVGEYS NLEVNASWTKERWNQEFTKHQVLIM TCYVALNVLKNGYLSLSDINLLVFD ECHLAILDHPYREIMKLCENCPSCP RILGLTASILNGKCDPEELEEKIQK LEKILKSNAETATDLVVLDRYTSQP CEIVVDCGPFTDRSGLYERLLMELE EALNFINDCNISVHSKERDSTLISK QILSDCRAVLVVLGPWCADKVAGMM VRELQKYIKHEQEELHRKFLLFTDT FLRKIHALCEEHFSPASLDLKFVTP KVIKLLEILRKYKPYERQQFESVEW YNNRNQDNYVSWSDSEDDDEDEEIE EKEKPETNFPSPFTNILCGIIFVER RYTAVVLNRLIKEAGKQDPELAYIS SNFITGHGIGKNQPRNKQMEAEFRK QEEVLRKFRAHETNLLIATSIVEEG VDIPKCNLVVRFDLPTEYRSYVQSK GRARAPISNYIMLADTDKIKSFEED LKTYKAIEKILRNKCSKSVDTGETD IDPVMDDDDVFPPYVLRPDDGGPRV TINTAIGHINRYCARLPSDPFTHLA PKCRTRELPDGTFYSTLYLPINSPL RASIVGPPMSCVRLAERVVALICCE KLHKIGELDDHLMPVGKETVKYEEE LDLHDEEETSVPGRPGSTKRRQCYP KAIPECLRDSYPRPDQPCYLYVIGM VLTTPLPDELNFRRRKLYPPEDTTR CFGILTAKPIPQIPHFPVYTRSGEV TISIELKKSGFMLSLQMLELITRLH QYIFSHILRLEKPALEFKPTDADSA YCVLPLNVVNDSSTLDIDFKFMEDI EKSEARIGIPSTKYTKETPFVFKLE DYQDAVIIPRYRNFDQPHRFYVADV YTDLTPLSKFPSPEYETFAEYYKTK YNLDLTNLNQPLLDVDHTSSRLNLL TPRHLNQKGKALPLSSAEKRKAKWE SLQNKQILVPELCAIHPIPASLWRK AVCLPSILYRLHCLLTAEELRAQTA SDAGVGVRSLPADFRYPNLDFGWKK SIDSKSFISISNSSSAENDNYCKHS TIVPENAAHQGANRTSSLENHDQMS VNCRTLLSESPGKLHVEVSADLTAI NGLSYNQNLANGSYDLANRDFCQGN QLNYYKQEIPVQPTTSYSIQNLYSY ENQPQPSDECTLLSNKYLDGNANKS TSDGSPVMAVMPGTTDTIQVLKGRM DSEQSPSIGYSSRTLGPNPGLILQA LTLSNASDGFNLERLEMLGDSFLKH AITTYLFCTYPDAHEGRLSYMRSKK VSNCNLYRLGKKKGLPSRMVVSIFD PPVNWLPPGYVVNQDKSNTDKWEKD EMTKDCMLANGKLDEDYEEEDEEEE SLMWRAPKEEADYEDDFLEYDQEHI RFIDNMLMGSGAFVKKISLSPFSTT DSAYEWKMPKKSSLGSMPFSSDFED FDYSSWDAMCYLDPSKAVEEDDFVV GFWNPSEENCGVDTGKQSISYDLHT EQCIADKSIADCVEALLGCYLTSCG ERAAQLFLCSLGLKVLPVIKRTDRE KALCPTRENFNSQQKNLSVSCAAAS VASSRSSVLKDSEYGCLKIPPRCMF DHPDADKTLNHLISGFENFEKKINY RFKNKAYLLQAFTHASYHYNTITDC YQRLEFLGDAILDYLITKHLYEDPR QHSPGVLTDLRSALVNNTIFASLAV KYDYHKYFKAVSPELFHVIDDFVQF QLEKNEMQGMDSELRRSEEDEEKEE DIEVPKAMGDIFESLAGAIYMDSGM SLETVWQVYYPMMRPLIEKFSANVP RSPVRELLEMEPETAKFSPAERTYD GKVRVTVEVVGKGKFKGVGRSYRIA KSAAARRALRSLKANQPQVPNSTRT RPLEQKLISEEDLAANDILDYKDDD DKV | Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining | Stability : Stable for 3 months from receipt of products under proper storage and handling conditions. | Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles. | Concentration : 50 μg/mL as determined by BCA | Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.