Active Recombinant Human ERBB2, His-tagged, Biotinylated

Specification :
| Description : Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2/neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.The primary amine of rh HER2 was labeled by biotin without decreasing its bioactivity, and has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination. | Source : HEK293 | Species : Human | Tag : His | Predicted N Terminal : Thr 23 | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Herceptin at 0.5 μg/mL (100 μL/well) can bind biotinylated HER2 with a linear range of 0.1-1 μg/mL when detected by HRP*-Labeled Streptavidin. | Molecular Mass : Biotinylated rh HER2 is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 72.4 kDa. The predicted N-terminus is Thr 23. DTT-reduced Protein migrates as 90-110 kDa in SDS-PAGE due to glycosylation.The primary amine of Biotinylated | Endotoxin : Less than 1.0 EU per μg of the Biotinylated rh HER2 by the LAL method. | Purity : >95% as determined by reduced SDS-PAGE. | Applications : Biotin Labeled Protein | Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).