Active Recombinant Human EGFR protein, Fc/Avi-tagged, Biotinylated

Specification :
| Description : Epidermal growth factor receptor (EGFR), also known as HER-1 and ErbB1, is a member of a subfamily of receptor tyrosine kinases comprised of four members:EGFR, ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD)containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosineautophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3-4). EGFRvIII is a tumor‐specificmutation that results from an in‐frame deletion removing 267 amino acids from the ECD and insertion of a glycine residue (5). EGFRvIII has a molecular mass ofapproximately 145 kDa and has been shown to have weaker activity than full-length EGFR (6). EGFR binds a subset of the EGF family ligands, including EGF,amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2,resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-9). EGFR can also be recruited to form heterodimers with the ligand_x005f_x0002_activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (7-9). EGFR isoverexpressed in a wide variety of tumors, with EGFRvIII overexpressed particularly in glioblastoma multiforme (GMB), and is the target of several anti-cancertherapeutics (5,10,11). Our Avi-tag Biotinylated Recombinant Human EGFRvIII features biotinylation at a single site contained within the Avi-tag, a unique 15 aminoacid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein isunchanged so there is no interference in the protein’s bioactivity. | Source : CHO cells | Species : Human | Tag : Fc/Avi | Predicted N Terminal : Leu25 | Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | Bio-activity : Measured by its binding ability in a functional ELISA.When Human EGFR antibody is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated RecombinantHuman EGFR Fc Chimera Avi-tag binds with an ED50 of 25-150 ng/mL. | Molecular Mass : 95-115 kDa, under reducing conditions | Protein length : Leu25-Ser378 | Endotoxin : | Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. | Applications : Bioactivity | Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. | Reconstitution : Reconstitute at 500 μg/mL in PBS.