Recombinant Human EGFRviii protein, His-Avi-tagged

Specification :
| Description : The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. | Source : HEK293 | Species : Human | Tag : His-Avi | Form : Supplied as 0.22um filtered solution in PBS (pH 7.4). | Molecular Mass : The protein has a predicted MW of 41.6 kDa .As a result of glycosylation, the protein migrates as 65-80 kDa under reducing (R) condition (Bis-Tris PAGE). | Protein length : It contains Leu 25 – Ser 645 (30-297: Val Cys Gln…Cys Pro Arg → Gly). | Endotoxin : Less than 0.05 EU per ug by the LAL method. | Purity : > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | Storage : The product should be stored at -70°C. Please do not repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.