Recombinant Human EGFR Protein, His-tagged

Specification :
| Description : The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] | Source : HEK293 cells | Species : Human | Tag : His | Molecular Mass : rhEGFR contains C terminal 6×His Tag and has a calculated MW of 69.5 kDa. The predicted N-terminal is Leu 25. In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa. | Endotoxin : Less than 0.01 ng endotoxin/1 µg rhEGFR. | Purity : >95% purity as determined by SDS-PAGE of reduced rhEGFR. | Notes : Research Use Only. Not for use in diagnostic or therapeutic procedures. | Storage : Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhEGFR should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles. | Storage Buffer : Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. | Publication : Discovery of Cyclic Peptidomimetic Ligands Targeting the Extracellular Domain of EGFR (2021)