Recombinant Human EGFRviii protein, His-tagged

Specification :
| Description : The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors; EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2–7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point | Source : HEK293 | Species : Human | Tag : His | Predicted N Terminal : Leu 25 | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. | Molecular Mass : The protein has a calculated MW of 40.5 kDa. The protein migrates as 60-70 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. | Protein length : Leu 25 – Ser 645, while the amino acids 30-297 are missing | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution. | Reconstitution : Reconstitute at 200 μg/mL in sterile deionized water.