Active Recombinant Human EGFR Protein (Leu 25 – Ser 645), His-tagged, low endotoxin

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested).Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested). | Molecular Mass : 70.5kDa | Protein length : Leu 25 – Ser 645 | Endotoxin : Less than 0.01 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Notes : Please avoid repeated freeze-thaw cycles. | Stability : No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution. | Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.