Active Recombinant Human EGFR protein, lFc-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : Llama IgG2b Fc | Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. | Bio-activity : Immobilized Human EGF, His Tag at 5 μg/mL (100 μL/well) can bind Human EGF R, Llama IgG2b Fc Tag with a linear range of 0.2-5 ng/mL.Immobilized Human EGF R, Llama IgG2b Fc Tag at 2 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.3-5 ng/mL.Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag. | Molecular Mass : This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 96.4 kDa. The protein migrates as 115-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. | Proteinlength : Leu25-Ser645 | Endotoxin : Less than 0.01 EU per μg by the LAL method. | Purity : >95% as determined by reduced SDS-PAGE. | Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.