Active Recombinant Human EGFR protein(Met1-Lys375), hFc-tagged

Specification :
| Source : Insect Cells | Species : Human | Tag : C-hFc | Protein length : Met1-Lys375 | Form : Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : 1. Immobilized EGF Protein, Human, Recombinant (ECD), HPLC-verified at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag) in functional ELISA. 2. Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag), the EC50 is 4-13 ng/mL. | Molecular Mass : The recombinant human EGFR consists of 589 amino acids and predicts a molecular mass of 65.1 kDa. | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.