Active Recombinant Human EGFR(T790M C797S L858R) protein, GST-tagged

Specification :
| Description : EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. | Source : insect cells | Species : Human | Tag : GST | Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5,150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mMDTT, 0.1mM PMSF, and 25% glycerol. | Bio-activity : The specific activity of EGFR (T790M C797S L858R) wasdetermined to be equivalent to 35 nmol/min/mg as per activityassay protocol. | Molecular Mass : 89~100kDa | Purity : >80% | Stability : 1yr at –70oC from date of shipment | Storage : Store product at –70oC. For optimal storage, aliquot targetinto smaller quantities after centrifugation and store atrecommended temperature. For most favorableperformance, avoid repeated handling and multiplefreeze/thaw cycles. | Concentration : 0.1 μg/μl