Active Recombinant Human ERBB2 Protein, Fc-tagged, Alexa Fluor 555 conjugated

Specification :
| Description : This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] | Source : HEK293 | Species : Human | Tag : Fc | Bio-activity : Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 Human breast carcinoma cell proliferation. The ED50 for this effect is typically 20-80 ng/mL in the presence of 0.6 μg/mL goat anti-hErbB2. | Molecular Mass : rhHER2-Fc was fused with Fc region of human IgG1 at C-terminal and has a calculated MW of 97.7 kDa. As a result of glycosylation, DTT-reduced protein migrates as 130-140 kDa polypeptide in SDS-PAGE. | N-terminal Sequence Analysis : Thr 23 | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. All lots are greater than 95 % pure. | Characteristic : Disulfide-linked homodimerLabeled with Alexa Fluor 555 via aminesWith an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. | Storage : Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhHER2-Fc should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles. | Storage Buffer : Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.