Active Recombinant Human ERBB2 Protein, Fc-tagged, Alexa Fluor 555 conjugated

Specification :
| Source : Mammalian cells | Species : Human | Tag : Fc | Form : Lyophilized | Bio-activity : Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED50 for this effect is typically 20-80 ng/mL in the presence of 0.6 μg/mL goat anti-hErbB2. | Molecular Mass : Recombinant Human ERBB2, Fc Chimera has a calculated MW of 96.8 kDa (monomer). In SDS-PAGE migrates as 125-130 kDa, reducing conditions220-250 kDa, non-reducing conditions. | N-terminal Sequence Analysis : Thr 23 | Purity : > 90 % by SDS-PAGE and analyzed by silver stain | Characteristic : Disulfide-linked homodimerLabeled with Alexa Fluor 555 via aminesWith an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. | Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade). | Storage Buffer : Lyophilized from a 0.2 μm filtered solution in PBS.