Recombinant Human ERBB2 Protein, His-tagged

Specification :
| Description : This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. | Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized | Molecular Mass : 70.2 kDa | Protein Length : 1255 | AA Sequence : MELAALCRWGLLLALLPPGAASTQV CTGTDMKLRLPASPETHLDMLRHLY QGCQVVQGNLELTYLPTNASLSFLQ DIQEVQGYVLIAHNQVRQVPLQRLR IVRGTQLFEDNYALAVLDNGDPLNN TTPVTGASPGGLRELQLRSLTEILK GGVLIQRNPQLCYQDTILWKDIFHK NNQLALTLIDTNRSRACHPCSPMCK GSRCWGESSEDCQSLTRTVCAGGCA RCKGPLPTDCCHEQCAAGCTGPKHS DCLACLHFNHSGICELHCPALVTYN TDTFESMPNPEGRYTFGASCVTACP YNYLSTDVGSCTLVCPLHNQEVTAE DGTQRCEKCSKPCARVCYGLGMEHL REVRAVTSANIQEFAGCKKIFGSLA FLPESFDGDPASNTAPLQPEQLQVF ETLEEITGYLYISAWPDSLPDLSVF QNLQVIRGRILHNGAYSLTLQGLGI SWLGLRSLRELGSGLALIHHNTHLC FVHTVPWDQLFRNPHQALLHTANRP EDECVGEGLACHQLCARGHCWGPGP TQCVNCSQFLRGQECVEECRVLQGL PREYVNARHCLPCHPECQPQNGSVT CFGPEADQCVACAHYKDPPFCVARC PSGVKPDLSYMPIWKFPDEEGACQP CPINCTHSCVDLDDKGCPAEQRASP LTSIISAVVGILLVVVLGVVFGILI KRRQQKIRKYTMRRLLQETELVEPL TPSGAMPNQAQMRILKETELRKVKV LGSGAFGTVYKGIWIPDGENVKIPV AIKVLRENTSPKANKEILDEAYVMA GVGSPYVSRLLGICLTSTVQLVTQL MPYGCLLDHVRENRGRLGSQDLLNW CMQIAKGMSYLEDVRLVHRDLAARN VLVKSPNHVKITDFGLARLLDIDET EYHADGGKVPIKWMALESILRRRFT HQSDVWSYGVTVWELMTFGAKPYDG IPAREIPDLLEKGERLPQPPICTID VYMIMVKCWMIDSECRPRFRELVSE FSRMARDPQRFVVIQNEDLGPASPL DSTFYRSLLEDDDMGDLVDAEEYLV PQQGFFCPDPAPGAGGMVHHRHRSS STRSGGGDLTLGLEPSEEEAPRSPL APSEGAGSDVFDGDLGMGAAKGLQS LPTHDPSPLQRYSEDPTVPLPSETD GYVAPLTCSPQPEYVNQPDVRPQPP SPREGPLPAARPAGATLERPKTLSP GKNGVVKDVFAFGGAVENPEYLTPQ GGAAPQPHPPPAFSPAFDNLYYWDQ DPPERGAPPSTFKGTPTAENPEYLG LDVPV | Purity : > 98% | Applications : WB; ELISA; FACS; FC | Stability : This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use. | Storage : At -20 centigrade. | Concentration : 1 mg/mL | Storage Buffer : PBS (pH 7.4-7.5). Sterile-filtered colorless solution. | Reconstitution : Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.