Recombinant Human ERBB2 protein(Met1-Thr652), Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : N/A | Protein length : Met1-Thr652 | Conjugation/Label : Biotin | Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : 1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD), Biotinylated at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 10-50 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM. | Molecular Mass : The recombinant human ERBB2 consists of 636 amino acids and predicts a molecular mass of 70 kDa. As a result of glycosylation, human ErbB2 migrates as an approximately 96.7 kDa protein in SDS-PAGE under reducing conditions. | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.