Active Recombinant Human Fas protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. | Bio-activity : Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 0.2-1.2 μg/mL in the presence of 200ng/mL recombinant human Fas ligand. | Molecular Mass : The secreted recombinant human FAS comprises 159 amino acids and has a predicted molecular mass of 18.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFAS is approximately 35 kDa due to glycosylation. | Protein Length : Met1-Asn173 | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.