Recombinant Human FAS protein

Specification :
| Description : Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. | Source : E.coli | Species : Human | Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. | Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2 ng/ml of rHuFas Ligand. | Molecular Mass : Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids. | Protein length : 157 | AA Sequence : RLSSKSVNAQVTDINSKGLELRKTV TTVETQNLEGLHHDGQFCHKPCPPG ERKARDCTVNGDEPDCVPCQEGKEY TDKAHFSSKCRRCRLCDEGHGLEVE INCTRTQNTKCRCKPNFFCNSTVCE HCDPCTKCEHGIIKECTLTSNTKCK EEGSRSN | Endotoxin : Less than 1 EU/μg of rHusFasR/TNFRSF6 as determined by LAL method. | Purity : >95% by SDS-PAGE and HPLC analysis. | Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. | Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.