Recombinant Human FASLG, His-tagged

Specification :
| Cat. No. : FASLG-2703H | Description : Fas ligand (FasL) is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Fas ligand is a homotrimeric protein and signals through trimerization of FasR, which spans the membrane of the “target” cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external serine matrix metalloproteinase MMP-7. Soluble FasL is less active than their membrane-bound counterparts and do not induce receptor trimerization and DISC formation. FasL is regarded as a potential target for immunotherapy. | Source : CHO cells. | Purity : Greater than 95% (determined by SDS-PAGE and HPLC analysis). | MW : 19.9 kDa. | Endotoxin Level : | Biological Activity : Determined by it”s ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50for this effect is 1 x 105units/mg. | Formulation : Lyophilized from a sterile-filtered solution with no additives. | Reconstitution : Please Note: Always centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use. | Storage : The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted sFasL should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.